T7 Exonuclease from New England Biolabs

Product Specs
ItemT7 Exonuclease
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0263S
Quantity1000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A method of large DNA fragment enrichment for nanopore sequencing in region 22q11.2Frontiers in GeneticsOctober 31, 2022Yu-Qing Lei, Liang-Pu Xu, Hua Cao, Xin-Rui Wangsamples were incubated at 60°C for 10 min and 80°C for 20 min. Ten units of T7 exonuclease (NEB, Cat. no. M0263) were then added and incubated at 25°C for 30 min. The targeted DNA was purified by 1× AMPure XP beads. Semi-nested

(2) Development of colorimetric sensors based on gold nanoparticles for SARS-CoV-2 RdRp, E and S genes detectionTalantaJune 1, 2022Ciro Rodríguez Díaz et al.the reaction. dsDNA products were treated with 0.4 U of T7 exonuclease (New England Biolabs; Cat. Nº M0263L) per μL of RT-PCR reaction during 45 min at 37 °C followed by 10 min at 80 °C to digest the unprotected

(3) Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNACellApril 28, 2022Balwina Koopal, Ana Potocnik, Sumanth K. Mutte, Cristian Aparicio-Maldonado, Simon Lindhoud, Jacques J.M. Vervoort, Stan J.J. Brouns, Daan C. Swarts,Thermo Scientific,EN0531 T4 polynucleotide kinase,Thermo Scientific,EK0031 T7 exonuclease,New England biolabs,M0263S ε-NAD,BIOLOG,N 010 (c)-(di/tri)NTPs,BIOLOG,Various ,, Critical commercial assays,Critical commercial

(4) Identification of Key Factors for Anoxic Survival of B. cenocepacia H111International Journal of Molecular SciencesApril 20, 2022Sarah Paszti, Alessandra Vitale, Yilei Liu, Rubina Braunwalder, Ratchara Kalawong, Olivier Biner, Gabriella Pessi, Leo Eberltransposon. Circularization followed by exonuclease treatment with ExoI (NEB, M0293L), T7 Gene 6 Exo (NEB, M0263S) and Lambda Exo (NEB, M0262S) occurred with size selected DNA fragments. DNA was sequenced (Miseq, Ilumina,

(5) Phosphorylation of the aggregate-forming protein alpha-synuclein on serine-129 inhibits its DNA-bending propertiesJournal of Biological ChemistryFebruary 1, 2022Sydney E. Dent, Dennisha P. King, Valerie R. Osterberg, Eleanor K. Adams, Marilyn R. Mackiewicz, Tamily A. Weissman, Vivek K. Unniremaining in the sample were removed using either T5 or T7 exonuclease (New England Biolabs, cat. # M0363, M0263) using the manufacturer’s recommended protocol. Electrophoretic mobility shift assay All recombinant

(6) Efficient acquisition of tens of thousands of short tandem repeats in single-cell whole-genome-amplified DNASTAR ProtocolsDecember 17, 2021Liming Tao, Zipora Marx, Ofir Raz, Ehud ShapiroExonuclease III (E. coli),NEB,Cat#M0206L RecJf,NEB,Cat#M0264L Exonuclease T,NEB,Cat#M0265L T7 Exonuclease,NEB,Cat#M0263L Lambda Exonuclease,NEB,Cat#M0262L NEBNext Ultra II Q5 MasterMix,NEB,Cat#M0544L MinElute PCR Purification

(7) Retrospective cell lineage reconstruction in humans by using short tandem repeatsCell Reports MethodsJuly 26, 2021Liming Tao et al.Exonuclease III (E.coli),NEB,Cat#M0206L RecJf,NEB,Cat#M0264L Exonuclease T,NEB,Cat#M0265L T7 Exonuclease,NEB,Cat#M0263L Lambda Exonuclease,NEB,Cat#M0262L NEBNext Ultra II Q5 MasterMix,NEB,Cat#M0544L MinElute PCR Purification

(8) ParB spreading on DNA requires cytidine triphosphate in vitroeLifeFebruary 20, 2020Adam SB Jalal, Ngat T Tran, Tung BK Leprotein",Exonuclease VII,NEB,Cat# M0379S,"10,000 units/mL" "Peptide, recombinant protein",Exonuclease T7,NEB,Cat# M0263S,"10,000 units/mL" "Chemical compound, drug",Benzonase nuclease,Sigma-Aldrich,Cat# E1014, "Chemical

(9) ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomesNucleic Acids ResearchMarch 16, 2018Hailong Wang et al.(T5exo; NEB, cat. no. M0363): 50°C for 30 min, then held at 4°C; T7 exonuclease (T7exo; NEB, cat. no. M0263): 25°C for 20 min, 50°C for 30 min, then held at 4°C; DNA polymerase I Klenow fragment (Kle; NEB, cat.

(10) Physiological protein blocks direct the Mre11–Rad50–Xrs2 and Sae2 nuclease complex to initiate DNA end resectionGenes & DevelopmentDecember 1, 2017Giordano Reginato, Elda Cannavo, Petr Cejkathose used for human RPA (Binz et al. 2006). T7 exonuclease was purchased from New England Biolabs (M0263). Yeast Sae2 was prepared as described previously (Cannavo and Cejka 2014) with the following modification:

(11) iDamIDseq and iDEAR: An improved method and computational pipeline to profile chromatin-binding proteinsDevelopmentNovember 15, 2016Jose Arturo Gutierrez-Triana, Juan L. Mateo, David Ibberson, Soojin Ryu, Joachim Wittbrodtbp to 2 kb). T7 exonuclease treatment CutSmart buffer (5 µl, 10×) , 10 units T7 exonuclease (NEB, M0263S) and 14 µl H2O were added to 30 µl of clean LM-PCR. The sample was incubated for 1 h at 25°C, cleaned

(12) Quality Control of Purified Proteins Involved in Homologous RecombinationMethods in Molecular BiologyJune 4, 2014Xiao-Ping Zhang, Wolf-Dietrich HeyerXhoI (NEB, R0146S, 20,000 Units/ml) and 10× buffer supplied by manufacturer. T7 Exonuclease (NEB, M0263S, 10,000 units/ml), a 5' → 3' exonuclease. Control enzyme. Exonuclease III (NEB, M0206S, 100,000 units/ml),

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T7 Exonuclease

T7 Exonuclease from New England Biolabs

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New England Biolabs

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T7 Exonuclease from New England Biolabs

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